An RNA species involved in Escherichia coli ribonuclease P activity. Gene cloning and effect on transfer RnA synthesis in vivo |
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Authors: | H Motamedi K Lee L Nichols F J Schmidt |
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Institution: | Department of Biochemistry University of Missouri-Columbia Columbia, Mo. 65212, U.S.A. |
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Abstract: | The isolation and characterization of a plasmid capable of complementing the temperature-sensitive transfer RNA biosynthetic mutation rnpA49 (ribonuclease P) is described. The DNA segment responsible for complementation codes for an RNA species, approximately 340 bases long. Hybridization-selection experiments indicated that all rnp mutants were deficient in the production of the complementing RNA at high temperature; these defects were not due to the accumulation of a precursor form of this RNA. Examination of tRNA species synthesized in vivo indicated that the plasmid clone did not completely relieve the deficiency in RNase P activity of rnpA49 since some tRNA precursors still accumulated in strain A49 containing the plasmid. At least one other tRNA precursor was no longer detectable in plasmid-containing rnpA49 cells. |
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Keywords: | Author to whom correspondence should be sent |
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