Analysis of lolB gene sequence and its use in the development of a PCR assay for the detection of Vibrio cholerae |
| |
| Authors: | Lalitha P Siti Suraiya M N Lim K L Lee S Y Nur Haslindawaty A R Chan Y Y Ismail Asma Zainuddin Zainul F Ravichandran M |
| |
| Affiliation: | School of Health Science, Universiti Sains Malaysia, Malaysia. |
| |
| Abstract: | A PCR assay has been developed based on a lolB (hemM) gene, which was found to be highly conserved among the Vibrio cholerae species but non-conserved among the other enteric bacteria. The lolB PCR detected all O1, O139 and non-O1/non-O139 serogroup and biotypes of V. cholerae. The analytical specificity of this assay was 100% while the analytical sensitivity was 10 pg/microL and 10(3) CFU/mL at DNA and bacterial level respectively. The diagnostic sensitivity and specificity was 98.5% and 100% respectively. |
| |
| Keywords: | Vibrio cholerae lolB PCR |
| 本文献已被 ScienceDirect PubMed 等数据库收录！ |
