An enzyme-linked immunoassay for lipoprotein lipase |
| |
| Authors: | J W Goers M E Pedersen P A Kern J Ong M C Schotz |
| |
| Institution: | Department of Chemistry, California Polytechnic State University, San Luis Obispo 93407. |
| |
| Abstract: | Polyclonal antibodies against bovine milk lipoprotein lipase (LPL) were used to generate an enzyme-linked immunosorbent assay (ELISA) for rat LPL. The antibodies to LPL were affinity purified on bovine LPL columns and were shown to be specific for LPL by immunoprecipitation and enzyme inhibition. The solid-phase ELISA was sensitive from 1.0 to 20 ng/ml of LPL and paralleled enzyme activity. Denatured rat LPL showed the same LPL mass as undenatured samples, allowing LPL mass to be quantitated effectively in a variety of rat tissue extracts. |
| |
| Keywords: | |
| 本文献已被 ScienceDirect 等数据库收录！ |
