Tissue culture propagation of Mentha spp. by the use of axillary buds |
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Authors: | E. L. Rech M. J. P. Pires |
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Affiliation: | (1) National Center for Genetic Resources, CENARGEN/EMBRAPA, P.O. Box 10.2372, 70.770 Brasília-DF, Brazil |
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Abstract: | A method is described for rapid multiplication from axillary buds of six Mentha species. Nodal segments from one-year old plants were grown on Murashige and Skoog medium (BMS), supplemented with BAP (1.0; 2.0 mg/l) and KIN (1.0 mg/l) and kept at 28 ± 1°C under fluorescent light for 30 days. After this period, several shoots (15–20 shoots per explant) with roots were produced which were placed in soil for further growth. |
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