云南汉族群体FIBRA、DHFRP2、ACTBP2基因座多态性及其法医学应用

采用Amp-FLP分型方法，调查云南汉族群体FIBRA、DHFRP2、ACTBP2基因座的遗传多态性，并将其应用于法医学实践。200份EDTA抗凝血采自昆明地区无血缘关系汉族个体，采用酚—氯仿法提取DNA；法医物证实际检案及亲子鉴定检材取自昆明医学院法医系物证教研室检案，各种动物血痕取自动物中心，采用酚—氯仿法或Chelex法提取DNA，PCR扩增，非变 性聚丙烯酰胺凝胶垂直板电泳，硝酸银染色分型。结果表明,FIBRA、DHFRP2、ACTBP2基因座分别观察到15、7、13个等位基因，基因型数分别是57、25、61。3个STR基因座的杂合度（H）分别为：0.8940、0.8174、0.9130;多态信息容量（PIC）分别是:0.8908、0.8045、0.9117；个人识别力（Dp）分别是：0.9733、0.9416、0.9772;非父排除率（Epp）分别是：0.7994、0.6542、0.8348，基因型频率分布均符合Hardy-Weinberg平衡。20个家系调查结果表明，3个基因组均符合孟德尔遗传规律。 Genetic Polymorphism of FIBRA，DHFRP2 and ACTBP2 and Their Forensic Application in Yunnan Han PopulationJING Qiang，NIE Sheng-jieDepartment of Forensic Medicine，Kunming Medical College，Yunnan Province 650031,ChinaAbstract:To investigate the genetic polymorphism of FIBRA，DHFRP2 and ACTBP2 in Yunnan Han population as well as their application in forensic science，EDTA-blood specimens were collected from 200 healthy individuals.The DNA were extracted either by the Chloro form,phenol method or by the Chelex-100 method.The PCR products were analyzed by PAG vertical electrophoresis,following by silver staining.All gene frequencies,discrimination power (DP),exclusion of paternity probability (EPP),heterozygosity (H),polymorphisms information content (PIC),matching probability (PM) as well as the Hardy-Weinberg test were calculated.The obtained data are beneficial in the understanding of population genetics of the three STR loci in Yunnan Han population and the results suggest that these loci are valuable genetic markers for paternity testing and personal identification in forensic science practice.Key words：short tandem repeat; Amp-FLP; genetic polymorphism

Genetic Polymorphism of FIBRA,DHFRP2 and ACTBP2 and Their Forensic Application in Yunnan Han Population

To investigate the genetic polymorphism of FIBRA,DHFRP2 and ACTBP2 in Yunnan Han population as well as their application in forensic science, EDTA-blood specimens were collected from 200 healthy individuals. The DNA were extracted either by the Chloro form, phenol method or by the Chelex-100 method. The PCR products were analyzed by PAG vertical electrophoresis, following by silver staining. All gene frequencies, discrimination power (DP) ,exclusion of paternity probability (EPP) ,heterozygosity (H) ,polymorphisms information content (PIC) ,matching probability (PM) as well as the Hardy-Weinberg test were calculated. The obtained data are beneficial in the understanding of population genetics of the three STR loci in Yunnan Han population and the results suggest that these loci are valuable genetic markers for paternity testing and personal identification in forensic science practice.