Pollen tube extract supports the movement of actin filaments in vitro |
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| Authors: | T Kohno T Okagaki K Kohama T Shimmen |
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| Institution: | (1) Department of Botany, Faculty of Science, University of Tokyo, Hongo, Tokyo;(2) The Physical Science Laboratories, Nishon University at Narashino, Funabashi, Chiba;(3) Department of Pharmacology, Gunma University, School of Medicine, Maebashi, Gunma;(4) Department of Life Science, Faculty of Science, Himeji Institute of Technology, 2167 Shosha, Himeji, 671-22 Hyogo, Japan |
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| Abstract: | Summary Muscle actin filaments labeled with rhodamine-phalloidin were observed to move on the surface coated with a crude extract of pollen tubes ofLilium longiflorum with an average velocity of 1.99±0.55  m/sec. The movement required both Mg2+ and ATP. These results indicate that the extract of pollen tubes contains a myosin-like translocatorAbbreviations ATP
adenosine-5 -triphosphate
- DTT
dithiothreitol
- EGTA
ethyleneglycol-bis-( -aminoethylether)N,N,N,N-tetraacetic acid
- PIPES
piperazine-N,N-bis-(2-ethanesulfonic acid)
- PMSF
phenylmethylsulfonyl fluoride |
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| Keywords: | Actin filaments Myosin Pollen tube |
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