VEGETATIVE PROPAGATION OF CHENOPODIUM QUINOA BY SHOOT TIP CULTURE |
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Authors: | M. Burnouf-Radosevich C. Paupardin |
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Affiliation: | Laboratoire de Physiologie Végétale (SN2), Université de Lille I, 59655 Villeneuve d'Ascq Cedex, France |
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Abstract: | An in vitro procedure is described for vegetative propagation of two varieties of Chenopodium quìnoa. Shoot tips of seedlings and adult plants were stimulated to produce multiple shoots by axillary branching in a B5 medium modified by decreasing sucrose (10 g/l), increasing nitrate (2,700 mg/l) and phosphate (315 mg/l) salts, and to which glycine was added (4 mg/l). This modified medium was supplemented with benzyladenine (0.22 mg/l) and naphthaleneacetic acid (0.018 mg/l). These conditions allowed development of more than twice the average number of shoots per culture as did the non-modified B5 medium. Absence of exogenous growth substances stimulated shoot elongation and rooting more effectively than auxins or gibberellic acid added to the medium. Rooted plants, transferred to a greenhouse, grew to 2 m at the flowering stage. |
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