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Production of bio-ethanol from soybean molasses by Saccharomyces cerevisiae at laboratory, pilot and industrial scales
Authors:Siqueira Paula F  Karp Susan G  Carvalho Júlio C  Sturm Wilerson  Rodríguez-León José A  Tholozan Jean-Luc  Singhania Reeta Rani  Pandey Ashok  Soccol Carlos R
Affiliation:Bioprocess Engineering and Biotechnology Division, Federal University of Paraná. P.O. Box 19 011, Curitiba, Brazil.
Abstract:The aim of this work was to develop an economical bioprocess to produce the bio-ethanol from soybean molasses at laboratory, pilot and industrial scales. A strain of Saccharomyces cerevisiae (LPB-SC) was selected and fermentation conditions were defined at the laboratory scale, which included the medium with soluble solids concentration of 30% (w/v), without pH adjustment or supplementation with the mineral sources. The kinetic parameters - ethanol productivity of 8.08g/Lh, Y(P/S) 45.4%, Y(X/S) 0.815%, m 0.27h(-1) and mu(X) 0.0189h(-1) - were determined in a bench scale bioreactor. Ethanol production yields after the scale-up were satisfactory, with small decreases from 169.8L at the laboratory scale to 163.6 and 162.7L of absolute ethanol per ton of dry molasses, obtained at pilot and industrial scales, respectively.
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