Differential Expression of NeuroD in Primary Cultures of Cerebral Cortical Neurons |
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| Authors: | Masahiro Katayama Ikuko Mizuta Yasuhiko Sakoyama Ayako Kohyama-Koganeya Kimio Akagawa Keiichi Uyemura Kazuhiro Ishii |
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| Affiliation: | aDepartment of Cell Biology, Institute for Virus Research, Kyoto University, Sakyo-ku, Kyoto, Japan;bDepartment of Genetics, Faculty of Medicine, Osaka University, Suita, Osaka, Japan;cDepartment of Physics, Faculty of Science, University of Tokyo, Tokyo, Japan;dDepartment of Physiology, Medical College, Kyorin University, Mitaka, Tokyo, Japan;eDepartment of Physiology, School of Medicine, Keio University, Shinjuku-ku, Tokyo, Japan |
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| Abstract: | We have investigated the expression patterns of a basic helix–loop–helix regulatory gene,neuroD,in primary cultures of murine cerebral cortical neurons. The differentiation states of neurons in primary cultures were determined by the sensitivity of neurons to glutamate toxicity and the expression of specific proteins such as the phosphorylated form of a 200-kDa neurofilament, HPC-1/syntaxin 1A, and cell adhesion molecule L1. The expression of neuroD was determined by RT-PCR analysis andin situhybridization. The experimental results thus obtained revealed that neuronal maturation is initiated between Day 7 and Day 11 in the culture as already known, and that the expression of neuroD decreases with increasing days in culture. Based on these findings, it was concluded that neuroD is expressed in immature neurons but not in mature ones. |
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