Effects of some steroid hormones on head-to-head association in bovine spermatozoa |
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Authors: | P E Lindahl |
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Affiliation: | Institute of Zoophysiology, University of Uppsala, S-75122 Uppsala, Sweden |
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Abstract: | In the presence of 2 × 10?6 M Ca2+ in Tris-buffered medium 0.5 × 10?6 M, oestradiol-17β or corticosterone significantly increased the head-to-head association of washed bull spermatozoa; in the same concentration, testosterone and 5α-dihydrotestosterone had no significant effects, whereas progesterone significantly dissociated the associated spermatozoa. At 8 × 10?6 M Ca2+ in the same medium, all five hormones increased the association to about the same level. In Tyrode solution with a Ca2+ concentration of 1.4 × 10?3 M, oestradiol-17β and corticosterone acted as above, whereas progesterone and the two testosterones effected dissociation. In Tyrode solution each of the dissociating hormones was combined with oestradiol-17β. In each case a sum of the effects of the two hormones was obtained without any stimulation or inhibition. All five hormones still produced significant effects at 5 × 10?7 M in Tyrode solution. A corresponding value for ATP was found at 1 × 10?5 M. |
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