Identification of aneuploids in Nicotiana tabacum by isozyme banding patterns

A biochemical system was devised to identify aneuploids of Nicotiana tabacum. Leaf tissue from 6 nullihaploids, 4 nullisomics, and 10 monosomics was analyzed electrophoretically on slab acrylamide gels. The staining systems used were for peroxidase, esterase, superoxide dismutase, malate dehydrogenase, and glutamate oxaloacetate transaminase. Nullihaploids and nullisomics could be distinguished from each other and from haploid or disomic types by their unique isozyme banding patterns. The banding patterns of the monosomics closely resembled those of the disomic. Morphologically similar aneuploids from different populations had similar isozyme banding patterns.This paper represents research completed in partial fulfillment of the requirements for the Ph.D. degree by the senior author. The investigations reported in this paper (No. 81-3-119) are in connection with a project of the Kentucky Agricultural Experiment Station, and the paper is published with the approval of the Director.