The cagA status of Helicobacter pylori isolates from dyspeptic children in Turkey |
| |
Authors: | Saltik Inci Nur Demir Hülya Engin Doruk Ertunç Ozge Darka Akyön Yakut Koçak Nurten |
| |
Affiliation: | Department of Pediatrics, Section of Gastroenterology, Hacettepe University, Faculty of Medicine, 06100 Ankara, Turkey. isaltik@hacettepe.edu.tr |
| |
Abstract: | There are inconsistent reports regarding cytotoxin-associated gene A (cagA) status of Helicobacter pylori isolates and the severity of the mucosal lesions in children. The aim of this study was to determine the prevalence of cagA(+) strains and to evaluate its correlation with clinic and endoscopic findings. We examined 45 H. pylori strains that were grown on brain-heart infusion agar supplemented with 7% horse blood. Following 72 h of incubation colonies were harvested and bacterial DNA was extracted. Polymerase chain reaction primers F1 and B1 were used to amplify a 348-bp internal fragment of cagA. The prevalence of cagA in Turkish pediatric patients was 55.6%. No association was found between cagA status and the severity of gastro-duodenal lesions. |
| |
Keywords: | Helicobacter pylori cagA Antral nodularity Children |
本文献已被 PubMed 等数据库收录! |