Mass spectrometric measurements of methane and oxygen utilization by methanotrophic bacteria |
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Authors: | Lars Joergensen Hans Degn |
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Institution: | Institute of Biochemistry, Odense University, Campusvej 55, DK-5230 Odense M, Denmark |
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Abstract: | Abstract A mass spectrometer with membrane inlet was used to measure methane and oxygen utilization rates at various methane concentrations in Methylosinus trichosporium and a locally isolated strain of a methane-oxidizing coccus (OU-4-1). The apparent K m for methane was found to be 2 μM for M. trichosporium and 0.8 μM for strain OU-4-1. These K m-values are 10–30 times lower than most previously reported values. The ratio of oxygen to methane utilization rates was 1.7 for M. trichosporium and 1.5 for strain OU-4-1 corresponding to a growth yield of 0.38 and 0.63 g dry weight/g methane, respectively. |
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Keywords: | Methane affinity methane oxidation Methylosinus trichosporium growth yield |
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