Two-dimensional polyacrylamide gel electrophoresis: an improved method for ribosomal proteins |
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Authors: | L J Mets L Bogorad |
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Affiliation: | Harvard University, The Biological Laboratories, 16 Divinity Avenue, Cambridge, Massachusetts 02138 USA |
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Abstract: | A two-dimensional electrophoresis system for analysis of ribosomal proteins with several advantages over previous systems is described. The general features of this system are: (1) first-dimension separation on the basis of mobility at pH 5.0 in 8 m urea and 4% polyacrylamide; (2) second-dimension separation on the basis of molecular weight using dodecyl sulfate detergent; (3) rapid electrophoretic shift between first- and second-dimension separation conditions; (4) high resolution separation can be obtained on 10-cm2 slabs with proteins from approximately 100 μg of ribosomal subunits; (5) capacity for handling up to 10 samples at a time, with electrophoresis complete within about 10 hr; and (6) the apparatus is relatively simple and inexpensive to construct and use. |
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