tRNAPhe cleavage by aminoglycosides is triggered off by formation of an abasic site |
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Authors: | Wrzesiński Jan Szczepanik Wojciech Ciesiołka Jerzy Jezowska-Bojczuk Małgorzata |
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Institution: | Institute of Bioorganic Chemistry, Polish Academy of Sciences, Noskowskiego 12/14, 61-704 Poznań, Poland. |
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Abstract: | This communication reports the characteristics of the mechanism of highly specific tRNA(Phe) cleavage, which occurs in the anticodon loop in the presence of aminoglycoside antibiotic-neomycin B. The data prove that the cleavage requires previous depurination of the polynucleotide chain at position 37, which is occupied by a hypermodified guanine base-wybutine. The results suggest that the phenomenon, previously considered as selective with respect to the presence of tRNA hypermodification, may concern far more RNA molecules, namely the ones carrying abasic sites. |
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Keywords: | Depurination Abasic site RNA cleavage tRNAPhe Aminoglycoside antibiotics Neomycin B |
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