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Potato tubers as a biofactory for recombinant antibodies |
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| Authors: | Artsaenko Olga Kettig Barbara Fiedler Ulrike Conrad Udo Düring Klaus |
| Institution: | (1) Institut für Pflanzengenetik und Kulturpflanzenforschung, Corrensstr. 3, 06466 Gastersleben, Germany;(2) Federal Centre for Breeding Research on Cultivated Plants, Institute for Breeding Methods in Vegetables, Neuer Weg 22/23, 06484 Quedlinburg, Germany;(3) Present address: Chemical and Biochemical Engineering, UMBC, Baltimore, MD 21228, USA;(4) Present address: Institut für Biotechnologie, Martin-Luther-Universität Halle-Wittenberg, Fachbereich Biochemie/Biotechnologie, Kurt-Mothes-Str. 3, 06120, Germany |
| Abstract: | Potato tubers have been successfully used for high-level production of a recombinant single-chain Fv (ScFv) antibody. Ubiquitous high-level expression was achieved under control of the CaMV 35S promoter through retention of the scFv protein in the endoplasmic reticulum. Recombinant antibodies accumulated up to 2% of total soluble tuber protein. After 1.5 years of tuber storage at 4 °C still half of the amount of scFv present in freshly harvested tubers was detectable. Its specific activity did not decrease during tuber storage. Recombinant protein could be efficiently purified from crude extracts by affinity chromatography. |
| Keywords: | antibody endoplasmic reticulum phytofarming potato production scFv |
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