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Iodination of proteins by IPy2BF4, a new tool in protein chemistry
Authors:Espuña Gemma  Andreu David  Barluenga José  Pérez Xavier  Planas Antoni  Arsequell Gemma  Valencia Gregorio
Institution:Proteomics Unit, Department of Experimental and Health Sciences, Universitat Pompeu Fabra, Dr. Aiguader 80, 08003 Barcelona, Spain.
Abstract:Iodination is a very useful method for protein characterization and labeling. However, derivatization chemistries used in most conventional iodination procedures may cause substantial alterations in protein structure and function. The IPy(2)BF(4) reagent bis(pyridine)iodonium (I) tetrafluoroborate] has been shown to be an effective iodinating reagent for peptides. Herein we report the first application of IPy(2)BF(4) in protein iodination in an aqueous medium using three representative substrates: insulin, lysozyme, and the enzyme 1,3-1,4-beta-d-4-glucanohydrolase. Our results show that IPy(2)BF(4) has clear advantages over existing methods in that the reaction is quantitative, fast, and selective for the most accessible Tyr residues of a protein, and it preserves the functional integrity of the protein when moderate Tyr labeling levels are pursued.
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