Synthesis of a low-molecular-weight form of exopolysaccharide by Bradyrhizobium japonicum USDA 110 |
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Authors: | Louch H A Miller K J |
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Institution: | Intercollege Graduate Program in Genetics, The Pennsylvania State University, University Park, Pennsylvania 16802, USA. |
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Abstract: | A novel extracellular low-molecular-weight polysaccharide was detected as a contaminant within extracellular cyclic beta-1,6-beta-1,3-glucan preparations from Bradyrhizobium japonicum USDA 110 cultures. Compositional analysis, methylation analysis, and nuclear magnetic resonance analysis revealed that this low-molecular-weight polysaccharide was composed of the same pentasaccharide repeating unit previously described for the high-molecular-weight form of the exopolysaccharide (EPS) synthesized by B. japonicum strains. Mass spectrometry analysis indicated that the size of this low-molecular-weight form of EPS was consistent with a dimeric form of the pentasaccharide repeating unit. |
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