Cloning and characterization of a regulatory gene of the SARP family and its flanking region from Streptomyces ambofaciens |
| |
Authors: | Culebras E Martínez E Carnero A Malpartida F |
| |
Institution: | Centro Nacional de Biotecnologia, CSIC, Campus Universidad Autónoma de Madrid, Spain. |
| |
Abstract: | In a search for activators of secondary metabolism we isolated a 12.6-kb DNA fragment from a genomic library of Streptomyces ambofaciens NRRL 2240 (the spiramycin producer ). Sequencing of 6 kb of the cloned fragment revealed a cluster of four ORFs (ORF1–4)
whose deduced products showed similarities to those of other genes involved in polyketide biosynthesis, including a pathway-specific
regulatory gene of the SARP family. The results of insertional inactivation of some of the cloned genes clearly indicate that
the isolated cluster does not code for spiramycin production, suggesting that some other polyketide compound might well be
produced by this strain.
Received: 14 May 1999 / Accepted: 28 July 1999 |
| |
Keywords: | |
本文献已被 PubMed SpringerLink 等数据库收录! |