溶菌酶中水分子的红外吸收光谱

本文报导了不同水含量的溶菌酶中水的红外吸收光谱的测量结果.室温下,在远离纯水OH带及OD带的两侧分别出现高频与低频带,峰的位移大小随水含量的不同而改变.低温下,当R(克水/克蛋白)>0.2后,比值A./A.随水含量增大而增大.本文对室温下高频及低频峰的可能原因及与水的冻结过程的联系进行了讨论.

IR ABSORPTION SPECTRA OF WATER MOLECULES IN LYSOZUME

The 1R absorption spectra of water in lysozyme film with various degree of hydration were recorded.At room temperature one high frequency band and one or two low freguency bands in both OH and OD regions are located.The frequency shift depends on the water content of lysozyme film. At low temperature radio A2420cm-1/A2480cm-1 raises with increase of water content, while R>0.2. The possible causes of high and low frequency bands and their relation to the freezing process were discussed.