| 人胃癌组织cDNA文库的建立 |
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| 引用本文： | 王易伦,孙仑泉,万芷芳,苏成芝.人胃癌组织cDNA文库的建立[J].中国生物化学与分子生物学报,1991,7(6):686-690. |
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| 作者姓名： | 王易伦 孙仑泉 万芷芳 苏成芝 |
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| 作者单位： | 第四军医大学生化教研室，第四军医大学生化教研室，第四军医大学生化教研室，第四军医大学生化教研室 西安 710032，西安 710032，西安 710032，西安 710032 |
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| 基金项目： | 国家自然科学基金(38870865) |
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| 摘 要： | 本文直接从一例人低分化胃腺癌组织中提取RNA和分离poly(A)~+RNA,进而合成双链cDNA。再以λgt10作为克隆载体和大肠杆菌C600hfl作为受体菌,构建了人胃癌cDNA文库。该文库中含有1.10×10~5重组子,重组率约为68.8％,克隆效率为2.21×10~6克隆/μgcDNA。
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| 关 键 词： | 人类 胃癌 cDNA 分子克隆 |
| 收稿时间： | 1991-12-20 |
Construction of Human Stomach Cancer cDNA Library |
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| Wang,Yi-lun Sun,lun-quan Wan,Zhi-fang Su,Cheng-zhi.Construction of Human Stomach Cancer cDNA Library[J].Chinese Journal of Biochemistry and Molecular Biology,1991,7(6):686-690. |
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| Authors: | Wang Yi-lun Sun lun-quan Wan Zhi-fang Su Cheng-zhi |
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| Institution: | (Department of Biochemistry, Fourth Military Medical University ,Xi'an 710032 |
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| Abstract: | Total RNA was extracted from a case of poorly differentiated adenoca-rcinoma of stomach and poly(A)+RNA was isolated, then, ds-cDNA was synthesized. Furthermore, using λt 10 system, we constructed the cDNA library of human stomach cancer tissue. The results showed that total 1.10×105 recombination was presented in that library, and the recombination rate and the cloning efficiency were-68.8% and 2.21×106 clones/μg cDNA respectively. |
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| Keywords: | Human Stomach cancer cDNA Molecular cloning |
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