Construction of an Effective Protein Expression System Using the <Emphasis Type="Italic">tpl</Emphasis> Promoter in <Emphasis Type="Italic">Escherichia coli</Emphasis>期刊界 All Journals 搜尽天下杂志传播学术成果专业期刊搜索期刊信息化学术搜索

Construction of an Effective Protein Expression System Using the tpl Promoter in Escherichia coli

Authors:	Takashi?Koyanagi,Takane?Katayama,Ai?Hirao,Hideyuki?Suzuki,Hidehiko?Kumagai author-information" > author-information__contact u-icon-before" > mailto:hidekuma@ishikawa-c.ac.jp" title=" hidekuma@ishikawa-c.ac.jp" itemprop=" email" data-track=" click" data-track-action=" Email author" data-track-label=" " >Email author

Affiliation:	(1) Division of Integrated Life Science, Graduate School of Biostudies, Kyoto University, Sakyo-ku Kyoto 606-8502, Japan;(2) Research Institute for Bioresources and Biotechnology, Ishikawa Prefectural University, Nonoichi-machi, Ishikawa 921-8836, Japan

Abstract:	An effective protein expression system was constructed in Escherichia coli using the promoter of the tyrosine phenol-lyase (tpl) gene of Erwinia herbicola. This system involves a mutant form of the TyrR protein with an enhanced ability to activate tpl and the TutB protein with an ability to transport L-tyrosine (an inducer of Tpl). The highest expression level obtained for this system was more than twice that obtained for the tac system, although it was lower than the level obtained for the T7 system, as revealed with the lac-reporter assay and SDS-polyacrylamide gel electrophoresis.

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