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Application of microarrays in high-throughput enzymatic profiling
Authors:Mahesh Uttamchandani  Xuan Huang  Grace Y J Chen  Lay-Pheng Tan  Shao Q Yao
Institution:(1) Department of Biological Sciences, National University of Singapore, 3 Science Drive 3, 117543 Singapore, Republic of Singapore;(2) Department of Chemistry, National University of Singapore, 3 Science Drive 3, 117543 Singapore, Republic of Singapore
Abstract:This review focuses on recent developments in microarray-based technologies for high-throughput screenings of enzymes. Novel methods of protein immobilization, detection of enzymatic activities, and inhibitions were highlighted.
Keywords:Microarray  proteomics  enzyme  inhibitors  fluorescence  immobilization
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