H (0) blood group determinant is present on soluble human L-selectin expressed in BHK-cells. |
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| Authors: | M Gohlke U Mach R Nuck B Volz C Fieger R Tauber W Reutter |
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| Institution: | Institut für Molekularbiologie und Biochemie der Freien Universit?t Berlin, Berlin-Dahlem, Germany. rolfnuck@zedat.fu-berlin.de |
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| Abstract: | In the present study we show that the H (0) blood group determinant Fuc alpha1-2Gal beta1-4GlcNAc beta1-R is present on N-linked glycans of soluble human L-selectin recombinantly expressed in baby hamster kidney (BHK) cells. The glycans were isolated using complementary HPLC techniques and characterized by a combination of exoglycosidase digestion and mass spectrometry. The linkage of the fucose residues was determined by incubation of the glycans with specific fucosidases. The H blood determinant Fuc alpha1-2Gal beta1-4GlcNAc beta1 was detected for bi-, 2,4 branched tri- and tetraantennary structures. To our knowledge, the proposed oligosaccharide structures represent a new glycosylation motif for recombinant glycoproteins expressed on BHK cells. |
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