The assessment of DNA from marine organisms via a modified salting-out protocol |
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Authors: | Giovanni Battista Ferrara Barbara Murgia Anna Maria Parodi Laura Valisano Carlo Cerrano Giulio Palmisano Giorgio Bavestrello Michele Sara |
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Institution: | (1) Dipartimento di Biologia, Università di Genova, Viale Benedetto XV, 16132 Genova, Italy;(2) Dipartimento per lo studio del Territorio e delle sue Risorse, Università di Genova, Corso Europa 26, 16132 Genova, Italy;(3) Dipartimento di Scienze del Mare, Università Politecnica delle Marche, Via Brecce Bianche, 60131 Ancona, Italy |
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Abstract: | We developed a rapid, practical and non-toxic salting-out method for the extraction of DNA from marine organisms, and tested
it on two representative species of Porifera and Cnidaria, both living in association with symbiotic zooxanthellae. We tested
the efficiency of the protocol by comparing the output of the method for fresh tissue, frozen tissue and tissue stored in
ethanol. It proved to be effective for extracting DNA in the case of the methods of preservation considered here, and for
obtaining quantities of DNA comparable to those obtained via the traditional approach. The DNA from both species was of good
quality. The DNA obtained was amplified by PCR using specific primers for the large ribosomal subunit, allowing the identification
of the presence of both the host and symbiont genomes. |
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Keywords: | Symbiodinium Porifera Cnidaria DNA PCR |
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