PHA production, from bacteria to plants. |
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| Authors: | H E Valentin D L Broyles L A Casagrande S M Colburn W L Creely P A DeLaquil H M Felton K A Gonzalez K L Houmiel K Lutke D A Mahadeo T A Mitsky S R Padgette S E Reiser S Slater D M Stark R T Stock D A Stone N B Taylor G M Thorne M Tran K J Gruys |
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| Institution: | Monsanto Company, Agricultural Sector, St. Louis, MO 63198, USA. |
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| Abstract: | The genes encoding the polyhydroxyalkanoate (PHA) biosynthetic pathway in Ralstonia eutropha (3-ketothiolase, phaA or bktB; acetoacetyl-CoA reductase, phaB; and PHA synthase, phaC) were engineered for plant plastid targeting and expressed using leaf (e35S) or seed-specific (7s or lesquerella hydroxylase) promoters in Arabidopsis and Brassica. PHA yields in homozygous transformants were 12-13% of the dry mass in homozygous Arabidopsis plants and approximately 7% of the seed weight in seeds from heterozygous canola plants. When a threonine deaminase was expressed in addition to bktB, phaB and phaC, a copolyester of 3-hydroxybutyrate and 3-hydroxyvalerate was produced in both Arabidopsis and Brassica. |
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