Analysis of the chromocenter DNA composition in polytene chromosomes of <Emphasis Type="Italic">Drosophila orena</Emphasis> ovarian nurse cells |
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Authors: | C E Usov T A Shelkovnikova V N Stegniy |
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Institution: | 1.Institute of Biology and Biophysics,Tomsk State University,Tomsk,Russia |
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Abstract: | Chromocenter DNA fragments of polytene chromosomes of Drosophila orena ovarian nurse cells were cloned from a region-specific library (Dore1) in a plasmid vector to yield 133 clones. A total of
76 clones were selected and sequenced. The total length of the sequenced fragments was 23940 bp. Analysis with several software
packages revealed various repetitive sequences among the fragments of the Dore1 library, including mobile genetic elements
(25 fragments homologous to various LTR retrotransposons, five fragments homologous to LINEs, three fragments homologous to
Helitrons, one fragment homologous to Polinton, and one fragment homologous to the mini-me non-LTR retrotransposon), four minisatellites, a satellite (SAR_DM), the (TATATG)n simple sequence repeat, and a low-complexity T-rich repeat. Sequences homologous to protein-coding genes were also found
in the Dore1 library. Various repetitive DNA sequences and gene homologs were identified as conserved sequences of pericentric
heterochromatin of polytene chromosomes of ovarian nurse cells in nine species of the melanogaster species subgroup. |
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