Characterisation of an amylase from a psychrotrophic Vibrio isolated from a deep-sea mud sample |
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Authors: | Tetsuo Hamamoto Koki Horikoshi |
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Institution: | Department of Biomedical Sciences and Technology and Biometry, Località Collemaggio, L'Aquila, Italy. |
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Abstract: | The gene coding for the class A beta-lactamase of Citrobacter diversus has been cloned and sequenced. It contains the information for a 294-amino-acid precursor protein, including a 27-residue N-terminal signal peptide. The deduced sequence of the N-terminal portion of the mature protein is in excellent agreement with that determined by microsequencing of the protein and readily explains the pI differences observed between the naturally occurring forms I and II of the enzyme. The sequence of the mature protein exhibits a very high degree of similarity with that of the Klebsiella oxytoca class A beta-lactamase. |
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Keywords: | Azospirillum nifHDK lacZ Gene fusion Immunoblotting |
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