Novel peptide inhibitors of myosin light chain kinase suppress the hyperpermeability of vascular endothelium |
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Authors: | A V Marchenko E O Stepanova A V Sekridova M V Sidorova V N Bushuev Zh D Bespalova V P Shirinsky |
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Institution: | 1.Russian Cardiology Research and Production Center,Moscow,Russia |
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Abstract: | The ability of novel cell-permeating peptide molecules derived from the peptide inhibitor of myosin light chain kinase (MLCK),
L-PIK (Arg-Lys-Lys-Tyr-Lys-Tyr-Arg-Arg-Lys) to inhibit this kinase in vitro and attenuate the thrombin-induced hyperpermeability
of endothelial cell monolayer in culture has been studied. It was found that compounds NαMeArg1]-L-PIK and Cit1]-L-PIK possess inhibitory activity towards MLCK comparable to that of L-PIK and the ability to suppress the hyperpermeability
of endothelium, whereas other modifications of L-PIK were less effective. Thus, among de novo synthesized peptides, NαMeArg1]-L-PIK and Cit1]-L-PIK demonstrate the inhibitory properties of the original peptide L-PIK and additionally surpass it in stability in blood
plasma. These peptides may be used in the design of novel antiedemic drugs. |
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