Regeneration of somatic hybrids of ginger via chemical protoplast fusion |
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Authors: | Qiuzhu Guan Yinghua Guo Yuxia Wei Fanzhen Meng Zhenxian Zhang |
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Institution: | (1) College of Agronomy and Biotechnology, China Agricultural University, 100193 Beijing, China;(2) Shandong Jining Agricultural Vocational School, 272100 Jining, Shandong, China; |
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Abstract: | Ginger (Zingiber officinale Rosc.) somatic hybridization was attempted by using polyethylene glycol (PEG)-mediated protoplast fusion. Protoplasts of
three ginger cultivars isolated from the embryogenic cell suspensions were fused with each other. The highest binary fusion
rate 13.5% in the fusion of ginger ‘Lushan Zhangliang jiang’ + ‘Chenggu Huang Jiang’ (LZ + CH)] was observed with the treatment
of 30% PEG6000 for 15 min. The three fusion combinations can efficiently develop into micro-colonies and redifferentiate,
but only the fusion of ginger ‘Chenggu Huang Jiang’ + ‘Sichuan Zhugen Jiang’ (CH + SZ) could regenerate plantlets. Approximately
15 months were used for the regeneration of whole plants, and 15 shoots were obtained from the fusion of LZ + CH. Three plantlets
were identified as hybrids by using RAPD, and they were all diploids by analysis with flow cytometry. |
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