Validation of endogenous controls for gene expression studies in peripheral lymphocytes from war veterans with and without PTSD期刊界 All Journals 搜尽天下杂志传播学术成果专业期刊搜索期刊信息化学术搜索

Validation of endogenous controls for gene expression studies in peripheral lymphocytes from war veterans with and without PTSD

Authors:	Jelena Brkljačić Nikola Tanić Danijela Vojnović Milutinović Ivana Elaković Sanja Manitašević Jovanović Tatjana Perišić Jadranka Dundjerski Gordana Matić

Affiliation:	(1) Department of Biochemistry, Institute for Biological Research "Siniša Stanković", University of Belgrade, 142 Despot Stefan Blvd., Belgrade, 11000, Serbia;(2) Department of Neurobiology, Institute for Biological Research "Siniša Stanković", University of Belgrade, 142 Despot Stefan Blvd., Belgrade, 11000, Serbia

Abstract:	Background Selection of appropriate endogenous control is a critical step in gene expression analysis. The aim of this study was to evaluate expression stability of four frequently used endogenous controls: β-actin, glyceraldehyde-3-phosphate dehydrogenase, β₂-microglobulin and RNA polymerase II polypeptide A in peripheral blood mononuclear cells from war veterans with and without posttraumatic stress disorder (PTSD). The study was designed as to identify suitable reference gene(s) for normalization of gene expression in peripheral blood mononuclear cells in response to war trauma and/or PTSD.

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