Ontogeny of d-Mannose Transport and Metabolism in Rat Small Intestine |
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Authors: | Mecedes Cano Anunciación A Ilundain |
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Institution: | 1. Departamento de Fisiología y Zoología, Facultad de Farmacia, Universidad de Sevilla, C/Profesor García González, no 2, 41012, Sevilla, Spain
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Abstract: | Oral mannose therapy is used to treat congenital disorders of glycosylation caused by a deficiency in phosphomannose isomerase.
The segmental distribution and ontogenic regulation of d-mannose transport, phosphomannose isomerase, and phosphomannose mutase is investigated in the small intestine of fetuses,
newborn, suckling, 1-month-old, and adult rats. The small intestine transports d-mannose by both Na+-dependent and Na+-independent transport mechanisms. The activities of both systems normalized to intestinal weight peak at birth and thereafter
they decreased. In all the ages tested, the activity of the Na+-independent mechanism was higher than that of the Na+/mannose transport system. At birth, the Na+-independent d-mannose transport in the ileum was significantly higher than that in jejunum. Phosphomannose isomerase activity and mRNA
levels increased at 1 month, and the values in the ileum were lower than in jejunum. Phosphomannose mutase activity in jejunum
increased during the early stages of life, and it decreased at 1 month old, as does the amount of mannose incorporated into
glycoproteins, whereas in the ileum, they were not affected by age. The phosphomannose isomerase/phosphomannose mutase activity
ratio decreased at birth and during the suckling period, and increased at 1 month old. In conclusion, intestinal d-mannose transport activity and metabolism were affected by ontogeny and intestinal segment. |
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