Donor-host mitochondrial compatibility improves efficiency of bovine somatic cell nuclear transfer |
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| Authors: | Zhong-hai Yan Yi-ye Zhou Jing Fu Fei Jiao Lei-wen Zhao Peng-fei Guan Shu-zhen Huang Yi-tao Zeng Fanyi Zeng |
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| Institution: | (1) Shanghai Institute of Medical Genetics, Shanghai Children's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, PR China;(2) Institute of Medical Science, Shanghai Jiao Tong University School of Medicine, Shanghai, PR China |
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| Abstract: | Background The interaction between the karyoplast and cytoplast plays an important role in the efficiency of somatic cell nuclear transfer
(SCNT), but the underlying mechanism remains unclear. It is generally accepted that in nuclear transfer embryos, the reprogramming
of gene expression is induced by epigenetic mechanisms and does not involve modifications of DNA sequences. In cattle, oocytes
with various mitochondrial DNA (mtDNA) haplotypes usually have different ATP content and can further affect the efficiency
of in vitro production of embryos. As mtDNA comes from the recipient oocyte during SCNT and is regulated by genes in the donor nucleus,
it is a perfect model to investigate the interaction between donor nuclei and host oocytes in SCNT. |
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