An easy screening assay for the enzymes of the Entner-Doudoroff pathway |
| |
| Authors: | K Kersters J De Ley |
| |
| Institution: | (1) Laboratory for Microbiology and Microbial Genetics, Facility of Sciences, State University, Gent, Belgium |
| |
| Abstract: | An easy and quick assay is described for the semiquantitative determination of the key enzymes of the Enter-Doudoroff pathway: gluconate-6-phosphate dehydrase and 2-keto-3-deoxygluconate-6-phosphate aldolase. It is a micro-method, based on the thin-layer chromatographical identification of the reaction products after incubation of cell-free extracts with both substrates.It is suitable for screening a large number of organisms. |
| |
| Keywords: | |
| 本文献已被 SpringerLink 等数据库收录！ |
|
