Selective precipitation of haptoglobin and alpha2-macroglobulin from human serum using Alocasia macrorhiza tuber protein |
| |
Authors: | Nayak B Shivananda Ulloor N Jagadish Shivaraj B |
| |
Affiliation: | Department of Biochemistry, Kasturba Medical College, Manipal-576 119, India. drbsraj@yahoo.co.in |
| |
Abstract: | Treatment of human serum with ammonium sulfate fraction (0-50%) of Alocasia macrorhiza tuber extract resulted in precipitation at neutral pH. The precipitate was dissolved at pH 10.5 and chromatographed on Sephadex G-100 column. Two protein peaks were resolved. While the first peak represented alpha2-macroglobulin and haptoglobin, the second peak accounted for specific Alocasia protein. Incidentally the Alocasia protein was shown to be responsible for selective and specific precipitation of alpha2-macroglobulin and haptoglobin from serum. Thus the plant protein in its pure form or in crude stage could be used for the rapid isolation of two of the prominent alpha2-globulins. |
| |
Keywords: | |
本文献已被 PubMed 等数据库收录! |
|