Characterization of rhodamine conjugated Agiotensin II peptide: synthesis,analysis and receptor binding and internalization |
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Authors: | Yadav Satya P Karnick Sadashiv Shen Wei-Zhen Zhang Jingli |
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Affiliation: | Biotechnology Biotechnology Core, The Lerner Research Institute, Cleveland Clinic Foundation, 9500 Euclid Avenue, Cleveland, Ohio 44195, USA. yadavs@ccf.org |
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Abstract: | The results in this study show that the rhodamine fluorophore can be specifically conjugated to Angiotensin II at Lys3 residue (substituted for a Val) without altering the biological activity of the parent compound. The conjugated peptide was characterized using HPLC, mass spectrometry, and N-terminal sequencing. The rhodamine-Angiotensin II binds effectively to AT1 receptor and gets internalized in clathrin coated vesicles by endocytosis. These results clearly suggest the usefulness of fluorophore-conjugated peptides in studies such as, ligand-receptor binding, and ligand-receptor complex internalization, for drug delivery using cell receptors and as an alternative to peptide hormone radioimmunoassays. |
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