Detection of intracellular cytokines during antioxidant supplementation in corticoid-dependent asthmatics and modulation of adhesion molecule expression on cultured endothelial cells |
| |
Authors: | M Horváthová E Jahnová F Gazdík |
| |
Institution: | (1) Department of Clinical Immunology, Institute of Preventive and Clinical Medicine, Limbová 14, Bratislava, Slovak Republic |
| |
Abstract: | In this study, we report on the interferon-γ (IFN-γ) and interleukin-4 (IL-4) cytokine responses to phorbol myristate acetate
(PMA)+ionomycin-stimulated CD3+ lymphocytes in asthmatic subjects when compared with normal donors. There was a significantly
lower production of intracellular IFN-γ in asthmatic patients. No difference was found for IL-4 production between these two
groups.
After administration of a multivitamin-mineral supplement containing selenium, zinc, vitamin A, vitamin B6, vitamin C, and vitamin E for 6 mo, a significant increase in the percentage of CD3+/IL-4 positive cells (p<0.05) was found.
The induction of endothelial cell adhesion molecule (CAM) expression in cultured human umbilical vein endothelial cells (HUVEC)
and whole-blood mixture was studied using flow cytometry. The ICAM-1 and VCAM-1 expressions were higher in the patients than
in control donors (p<0.05). There is a correlation between the increased percentage of CD3+/IFN-γ positive cells and reduced endothelial ICAM-1
and VCAM-1 expression after 6 mo of intervention period. No apparent effect of supplementation on CAM expression was found,
suggesting that these changes do not arise from an antioxidant mechanism. This newly developed whole-blood technique for the
assessment of CAM expression can be of use for monitoring therapy in inflammatory diseases. |
| |
Keywords: | Intracellular cytokines endothelial cells cell adhesion molecules asthma antioxidants flow cytometry |
本文献已被 SpringerLink 等数据库收录! |
|