| 氧化硅包裹的磁性纳米粒子纯化质粒DNA |
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| 引用本文： | 吴能表,薛莲,陈丽娜,曾献生,盛蓉生.氧化硅包裹的磁性纳米粒子纯化质粒DNA[J].中国生物化学与分子生物学报,2009,25(10):958-962. |
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| 作者姓名： | 吴能表 薛莲 陈丽娜 曾献生 盛蓉生 |
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| 作者单位： | 1)西南大学生命科学学院，重庆400715；2）西南大学化学化工学院，重庆400715；3）卡南吉医药科技（上海）有限责任公司，上海201203 |
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| 摘 要： | 质粒的分离纯化在分子生物学实际工作中占有重要地位.本文采用氧化硅包裹的磁性纳米粒子，平均粒径为20 nm左右,在外加磁场的作用下,从细胞粗提掖中快速分离质粒DNA.用这种方法成功地从大肠杆菌DH5α浓缩和纯化得到了pUC19质粒，该质粒具有生物活性，可直接用于限制性酶切和细胞转化等分子生物学下游操作.
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| 关 键 词： | 氧化硅包裹的磁性纳米粒子 质粒DNA 分离纯化 |
| 收稿时间： | 2009-4-13 |
| 修稿时间： | 2009-5-25 |
Isolation and Purification of Plasmid DNA by Silica-coated Magnetic Nanoparticles |
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| WU Neng-Biao,XUE Lian,CHEN Li-Na,ZENG Xian-Sheng ,SHENG Rong-Sheng.Isolation and Purification of Plasmid DNA by Silica-coated Magnetic Nanoparticles[J].Chinese Journal of Biochemistry and Molecular Biology,2009,25(10):958-962. |
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| Authors: | WU Neng-Biao XUE Lian CHEN Li-Na ZENG Xian-Sheng SHENG Rong-Sheng |
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| Institution: | 1) School of Life Sciences, Southwest University, Chongqing 400715, China;
2) School of Chemistry, Southwest University, Chongqing 400715, China;3)Challenger MediTech Company, Ltd, 301-1, 326 Edison Road, Zhangjiang HighTech Park, Shanghai 201203, China
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| Abstract: | To obtain high-quality purified plasmid DNA is critical for experimental studies in molecular biology and biomolecular analysis. We have developed a simple and reliable method for the rapid isolation of plasmid DNA from crude cell lysates with silica-coated magnetic nanoparticles with an average diameter of 20 nm. pUC19 plasmid were successfully enriched and purified from the E. coli DH5α under the magnetic fields. The obtained plasmid retained native the bioactivity to be used for restriction enzyme digestions and cell transformations. |
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| Keywords: | silica-coated magnetic nanoparticles plasmid DNA isolation and purification |
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