金葡菌L型感染在卵巢癌中检测的意义

目的探讨金葡菌L型感染在卵巢癌中检测的意义。方法应用革兰染色、免疫组织化学染色技术检测HO-8910PM细胞株以及97例卵巢乳头状癌、23例卵巢乳头状瘤石蜡包埋组织中细菌L型的感染情况。结果（1）HO-8910PM细胞与金葡菌L型体外共培养后在肿瘤细胞的胞浆及胞核中检测到L型的阳性表达。（2）卵巢乳头状癌和乳头状瘤组织中,细菌L型感染率分别为25.8%（25/97）和13.0%（3/23）。L型检出率与卵巢癌的临床分期、病理分级及腹腔淋巴结转移的差异均有显著性（P〈0.005）,与组织学类型无关（P〉0.05）。结论金葡菌L型可以进入细胞内,并能够在一定程度上促进卵巢癌的发生、发展。

Detection of Staphylococcus aureus L-form infection in ovarian carcinoma and its significance

Objective To detect infection of Staphylococcus aureus L-forms in ovarian neoplasms,and discuss its significance in ovarian neoplasms. Method Cram's staining and immunohistochemical technology were used for detection of bacterial L-forms in the infected HO-8910PM cell line, 97 cases of ovarian papillary carcinoma cell and 23 cases of benign tumor cell. Result (1) Signals of L-forms were detected in both cytoplasms and nuclei of HO-8910PM cell culture. (2) Ratios of L-form infection in malignant and benign neoplasms of ovary were 25.8% and 13.0% respectively. The positive rate of L-forms increased according to the clinical stage and pathological grade (P < 0.005) , and was higher in patients with celiac lymph nodes involvement (P < 0.005). Conclusion Infection of Staphylococcus aureus L-forms can promote occurrence and development of ovarian carcinomas.