Identification of 2Fe-2S cysteine ligands in putidaredoxin |
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Authors: | N C Gerber T Horiuchi H Koga S G Sligar |
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Institution: | Department of Biochemistry, University of Illinois, Urbana 61801. |
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Abstract: | The iron-sulfur center of putidaredoxin is coordinated by four cysteine sulfhydrals. In order to determine which of the six cysteine residues in the protein coordinate the Fe-S center, we have individually mutated cysteine residues 73, 85 and 86 into serines. Of these mutant proteins, only C85S and C73S express holo-protein as evidence by SDS-PAGE and EPR spectroscopy. This leads us to the conclusion that residues 39,45,48, and 86 are the cysteines that coordinate the iron-sulfur center in putidaredoxin. |
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