Microflora of dissimilative nitrate reduction in a denitrifying reactor

The predominant denitrifiers and ammonifiers from methanogenic, aerobic and denitrifying reactor sludge were isolated and characterised. The population of ammonifiers increased by three orders of magnitude during the operation of the denitrifying reactor treating landfill leachate. The predominant ammonifiers were enterobacteria, and the predominant denitrifiers belonged to the genera Alcaligenes and Pseudomonas. Studies in pure culture showed that ammonia production by ammonifiers was favoured by fermentable substrates and by high C/N ratios. For acetate, only nitrite was obtained as the reduction product of nitrate, even at high C/N ratios. Furthermore, for glucose, nitrite addition caused a shift in fermentation products, with an increase in the acetate/ethanol ratio, with no significant differences in growth rates. Received: 14 January 1998 / Received revision: 11 May 1998 / Accepted: 21 May 1998