Enhancement of topoisomerase I-mediated unwinding of supercoiled DNA by the radioprotector WR-33278 |
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| Authors: | E A Holwitt E Koda C E Swenberg |
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| Affiliation: | Radiation Biochemistry Department, Armed Forces Radiobiology Research Institute, Bethesda, Maryland 20814-5145. |
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| Abstract: | The radioprotector WR-33278, the disulfide of WR-1065 (N-(2-mercaptoethyl)-1,3-diaminopropane), is shown to stimulate eukaryotic topoisomerase I unwinding of negatively supercoiled DNA. This observation suggests the possibility that some protection may be conferred to DNA either by a decrease in its supercoiled state or by altering directly other enzymatic processes. This is the first report of a radioprotective compound stimulating an enzyme involved in DNA structure and synthesis. |
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