Inhibitor of anion transport, DIDS, releases Ca2+ from hepatic microsomes |
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| Authors: | S Kimura B C Robison N Kraus-Friedmann |
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| Institution: | Department of Physiology and Cell Biology, University of Texas Health Science Center, School of Medicine, Houston 77225. |
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| Abstract: | Addition of 4,4'-diisothiocyanostilbene-2, 2'-disulfonic acid (DIDS) to Ca2+ loaded hepatic microsomal vesicles evoked a dose-dependent release of the accumulated Ca2+. Ca2+ uptake was also inhibited. The effects of DIDS do not seem to be due to the inhibitions of either Cl- or proton fluxes. The results indicate that DIDS inhibits Ca2+ uptake and releases Ca2+ by inhibiting the Ca2+-ATPase and the formation of the phosphorylated intermediate of the enzyme, and that it might interact with a specific site on the vesicle which is involved in the translocation of Ca2+ across the microsomal and mitochondrial membranes. |
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