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| 新疆胀果甘草总黄酮的细胞毒活性研究 | |
| 引用本文： | 郭霞,盛磊,木合布力·阿布力孜,阿布力孜·阿布杜拉. 新疆胀果甘草总黄酮的细胞毒活性研究[J]. 天然产物研究与开发, 2011, 23(1): 49-53 |
| 作者姓名： | 郭霞 盛磊 木合布力·阿布力孜 阿布力孜·阿布杜拉 |
| 作者单位： | 1. 新疆医科大学,新疆地方病分子生物学重点实验室 2. 新疆医科大学,药学院药化有机教研室 3. 新疆医科大学基础医学院生物化学暨分子生物学教研室,乌鲁木齐,830011 |
| 基金项目： | 新疆维吾尔自治区重点实验室基金 |
| 摘 要： | 为了探讨新疆胀果甘草总黄酮(general flavonoids,GFs)对宫颈癌细胞的细胞毒活性,自制备胀果甘草GFs,通过离子阱喷雾式质谱技术分析,建立GFs组分的指纹图谱;并且对SiHa宫颈癌细胞进行GFs药物干预,应用MTT法和流式细胞技术分别鉴定细胞活力和细胞凋亡率。获得了新疆胀果甘草GFs组分的特征性指纹图谱,提取物中GFs含量可达35.40%;发现在0～500μg/mL GFs浓度范围内,GFs药物干预引起细胞活力梯度性下降,其MTT法检出的最低值为12%;在0～1000μg/mL GFs浓度范围内,GFs诱导的细胞凋亡率呈现梯度性上升趋势,其流式细胞仪检出的最高值为78%。结果表明新疆胀果甘草GFs具有较强的细胞毒活性,能够抑制宫颈癌细胞生长与活力,并诱导细胞凋亡。 |
| 关 键 词： | 新疆胀果甘草 总黄酮 细胞毒活性 |
Cytotoxic Activity of Flavonoids from Xinjiang Glycyrrhiza inflata Bat. |
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| GUO Xia,SHENG Lei,Mourboul ABLISE,Abulizi ABUDULA. Cytotoxic Activity of Flavonoids from Xinjiang Glycyrrhiza inflata Bat.[J]. Natural Product Research and Development, 2011, 23(1): 49-53 | |
| Authors: | GUO Xia SHENG Lei Mourboul ABLISE Abulizi ABUDULA |
| Affiliation: | GUO Xia1,SHENG Lei1,Mourboul ABLISE2,Abulizi ABUDULA31Xinjiang Key Laboratory of Molecular Biology and Endemic Diseases,2Department of Pharmaco-chemistry,3Department of Biochemistry and Biology,Basic Medical College,Xinjiang Medical University,Urumqi 830011,China |
| Abstract: | To investigate the cytotoxic activity of general flavonoids(GFs) from Xinjiang Glycyrrhiza inflata Bat,on cervical carcinoma cells.GFs was prepared from Glycyrrhiza inflata,and the finger print map was characterized by Electro Spray Mass Spectrometry;SiHa cervical carcinoma cells were treated with GFs,and the cell viability and cellular apoptosis were determined by MTT method and flow cytometry,respectively.We discovered that GFs from Xinjiang Glycyrrhiza inflata had special finger print characteristics;the... |
| Keywords: | Xinjiang Glycyrrhiza inflata general flavonoids(GFs) cytotoxic activity |
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