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| 一株嗜盐菌新种的分离与鉴定 | |
| 引用本文： | 白雪冬,吴敏,李遂焰. 一株嗜盐菌新种的分离与鉴定[J]. 天然产物研究与开发, 2011, 23(1): 6-9 |
| 作者姓名： | 白雪冬 吴敏 李遂焰 |
| 作者单位： | 1. 西南交通大学生命科学与工程学院,成都,610031;浙江大学生命科学学院,杭州,310058 2. 浙江大学生命科学学院,杭州,310058 3. 西南交通大学生命科学与工程学院,成都,610031 |
| 基金项目： | 国家重点基础研究发展规划资助项目(973计划) |
| 摘 要： | 从舟山册子岛船舶压载水泥样中分离到一株细菌S3-22,其与已知细菌的16S rDNA序列相似性低于97%,G+C mol%为54.9 mol%,主要脂肪酸iso-C17:1ω9c(24.99%),细胞醌型为甲基萘醌MK-5。革兰氏染色阴性,最适生长条件为30～37℃、pH7、3%NaCl。嗜盐,氧化酶、接触酶、淀粉酶、酯酶呈阳性,可还原硝酸盐。依据其16S rDNA序列相似性、系统发育学分析及细胞与分子水平的鉴定表明,该菌是Kordiimonas属的一个新种,菌株S3-22的16S rDNA序列登陆号为FJ847942。 |
| 关 键 词： | 嗜盐菌 新种 鉴定 |
Seperation and Identification of a Novel Halophile |
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| BAI Xue-dong,WU Min,LI Sui-yan. Seperation and Identification of a Novel Halophile[J]. Natural Product Research and Development, 2011, 23(1): 6-9 | |
| Authors: | BAI Xue-dong WU Min LI Sui-yan |
| Affiliation: | BAI Xue-dong1,2,WU Min2,LI Sui-yan11College of Life Science and Engineering,Southwest Jiaotong University,Chengdu 610031,China,2College of Life Science,Zhejiang University,Hangzhou 310058 |
| Abstract: | A marine bacterium,designated strain S3-22,was isolated from the ballast water sample from Zhoushan archipelago,China.16S rDNA sequences similarity of strain S3-22 to all recognized bacterial species was not greater than 97%.The DNA G+C content was 54.9 mol%.The dominant fatty acid was iso-C17:1ω 9c(24.99%).The major respiratory quinine was MK-5.Cells of strain S3-22 were gram-negative.Optimal growth of strain S3-22 was at pH 7.0,30–37 ℃ and 3%(w/v) NaCl.It was halophilic and tested positive for catalase,oxidase,amylase,and esterase.In addition,it could deoxidize the nitrate.Based on the evidence of 16S rDNA sequences similarities,phylogenetic analysis and cellular and molecular level identification,the halophile formed a phyletic lineage which was distinct from the known Kordiimonas species.Strain S3-22 represented a novel species in the Kordiimonas,its Genebank Accession number was FJ847942. |
| Keywords: | halophile novel identification |
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