| 海蜇胶原蛋白的提取及纯化(英文) |
| |
| 引用本文： | 刘磊,陈伟.海蜇胶原蛋白的提取及纯化(英文)[J].天然产物研究与开发,2011,23(1):72-75. |
| |
| 作者姓名： | 刘磊 陈伟 |
| |
| 作者单位： | 江南大学医药学院分子生物学实验室,无锡,214122 |
| |
| 摘 要： | 通过胃蛋白酶的限制性酶切、选择性盐析及阳离子交换柱的层析作用,低温从海蜇中胶层提取可得到胃蛋白酶促溶且去除端肽(telopeptide)的胶原蛋白(PSC)。紫外-可见扫描测得PSC的最大吸收峰位于235nm。凝胶电泳及氨基酸分析显示这些都符合Ⅰ型胶原的特征,同水母S.nomurai和Rhopilema asamushi来源的胶原蛋白基本一致,作为一个低等动物,其氨基酸分析显示了脊椎动物和非脊椎动物的微小差异。
|
| 关 键 词： | 水母 胶原 提取 纯化 氨基酸组成 |
Extraction and Isolation of Pepsin-solubilised Collagen from Jellyfish (Rhopilema esculentum ) |
| |
| LIU Lei,CHEN Wei.Extraction and Isolation of Pepsin-solubilised Collagen from Jellyfish (Rhopilema esculentum )[J].Natural Product Research and Development,2011,23(1):72-75. |
| |
| Authors: | LIU Lei CHEN Wei |
| |
| Institution: | LIU Lei,CHEN Wei Laboratory of molecular biology,School of Medicine and Pharmaceutics,Jiangnan University,Wuxi 214122,China |
| |
| Abstract: | Pepsin-solubllised collagen (PSC) which was isolated from the mesogloea of jellyfish (Rhopilema esculentumn),by limited pepsin digestion and isolated by selective precipitation with NaCl and cm-cellulose chromatography.The PSC exhibited a maximum absorbance at 235 nm.The results of SDS-PAGE and amino acid compositions suggested the purified collagen was type Ⅰ collagen.It was the same with those of the collagen from jellyfish umbrella and the acidsoluble collagen from its mesogloe,such as S.nomurai and Rhopilema asamushi.As a primitive animal,amino analysis of psc revealed the slight difference between invertebrate and vertebrate. |
| |
| Keywords: | jellyfish collagen extraction isolation amino acid compositions |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
