Simultaneous analyses of phenethylamine, phenylethanolamine, tyramine and octopamine in rat brain using fluorescamine |
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| Authors: | Shinkichi Kamata Kenji Imura Akira Okada Yasunaru Kawashima Atsushi Yamatodani Takehiko Watanabe Hiroshi Wada |
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| Institution: | First Department of Surgery, Osaka University Medical School, Kita-ku, Osaka Japan;Second Department of Pharmacology, Osaka University Medical School, Kita-ku, Osaka Japan |
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| Abstract: | A fluorometric method for the simultaneous analyses of phenethylamine, phenylethanolamine, tyramine and octopamine has been developed. The method involves ion-exchange chromatography, derivatization with fluorescamine, solvent extraction and then separation by thin-layer chromatography. The fluorescent spots are then quantitated by scanning. The detection limits of this method are about 10 pmoles for phenethylamine, phenylethanolamine and tyramine, and 20 pmoles for octopamine. The method was used for simultaneous analyses of putative neurotransmitter amines in whole rat brain. |
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