小茴香果实中总黄酮含量测定方法研究

目的：测定小茴香果实中的总黄酮含量,以利于后续的提取操作。方法：采用分光光度法,以芦丁为标准样品,测定小茴香中的总黄酮含量;通过单因素和正交试验确定果实中总黄酮的最佳提取条件。结果：总黄酮的最佳提取条件为：乙醇浓度70%,提取时间2h,料液比1∶30,提取温度70℃,在此条件下提取率可达2.871。结论：试验结果可能为以后小茴香资源的开发利用提供重要的理论依据。

Study on the Total Flavonoids Contents in Foeniculum vulgare Mill

Objective： To simplify the extraction of flavonoid in Foeniculum vulgate Mill. by using spectroscopic method. Methods： The total flavonoids content of F.vulgare were measured by using spectroscopic methods with rutin as a stan- dard. The extraction technology of total flavonoids of Foeniculum vulgare Mill. was optimized by using single factor and or- thogonal test. Results： The optimal extraction condition was determined as follows： 70% ethanol which has 1：30 solid liquid ratio and heat at 70℃ refux for 2 hours, and the absorbance of the total flavonoids extraction is 2.871 under the optimal condition. Conclusion： Results can provide important theoretical basis for exploitation of F.vulgare Mill. resources.