苯污染对大鼠脑组织环核苷酸与相关蛋白水平的影响

为了探索苯污染对人类健康损伤作用的因素, 选用SPF级Wistar大鼠为实验对象, 分为4组: 低剂量组灌胃苯 0.19 g·kg-1, 中剂量组灌胃苯 0.38 g·kg-1, 高剂量组灌胃苯 0.76 g·kg-1, 对照组灌胃菜籽油2 mL·kg-1;用酶联免疫吸附法(ELISA)检测脑组织中环核苷酸和相关蛋白的水平。结果发现, 连续给予大鼠不同剂量苯21 d, 低、中、高剂量大脑皮质cAMP、c-fos、Bax和caspase-9水平比对照组分别升高了22.41%-59.53%, 38.28%-81.34%, 29.08%-63.04%和44.53%-70.07%; cGMP、Bcl-2水平比对照组分别降低了15.20%-49.79% 和13.42%-46.98%。丘脑组织cAMP、c-fos、Bax和caspase-9水平比对照组分别升高了34.71%-63.34%, 28.32%-78.04%, 16.54%-49.46% 和9.10%-61.57%; cGMP、Bcl-2水平比对照组分别降低了20.00%-33.83% 和8.53%-39.53%。结果提示, 苯污染引起脑组织损伤和细胞凋亡主要与cAMP、c-fos、Bax、caspase-9水平升高及cGMP和Bcl-2水平降低有关。

Effects of benzene pollution on the levels of cyclic nucleotides and related proteins in rat brain tissue

In order to study the factors of benzene pollution on human health,SPF Wistar rats were selected as experimental materials and divided into four groups:the low-dose group was treated with benzene 0.19 g·kg^-1;the medium-dose group was treated with benzene 0.38 g·kg^-1;the high-dose group was treated with benzene 0.76 g·kg^-1;and the control group was treated with canola oil 2 mL·kg^-1.The levels of cyclic nucleotides and related proteins in brain tissue were detected by enzyme-linked immunosorbent assay(ELISA).The results showed that the levels of cAMP,c-fos,Bax and caspase-9 in the cerebral cortex of the rats were increased by 22.41%-59.53%,38.28%-81.34%,29.08%-63.04%and 44.53%-70.07%,respectively,when the rats were treated with different doses of benzene for 21 days.cGMP and Bcl-2 levels were reduced by 15.20%-49.79%and 13.42%-46.98%,respectively,compared with the control group.The levels of cAMP,c-fos,Bax and caspase-9 in thalamic tissues were increased by 34.71%-63.34%,28.32%-78.04%,16.54%-49.46%and 9.10%-61.57%,respectively,compared with the control group.The levels of cGMP and Bcl-2 decreased by 20.00%-33.83%and 8.53%-39.53%,respectively,compared with the control group.The results indicated that the brain tissue damage and cell apoptosis caused by benzene pollution were mainly related to the increased levels of cAMP,c-fos,Bax,caspase-9 and the decreased levels of cGMP and Bcl-2.