Quantitative evaluation of two pathways for phosphatidylcholine biosynthesis in rat brain in vivo |
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| Authors: | P Orlando G Arienti F Cerrito P Massari G Porcellati |
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| Institution: | (1) Department of Pharmacology, The Catholic University, Rome, Italy;(2) Department of Biochemistry, University of Perugia, Perugia, Italy |
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| Abstract: | Me-3H] choline and 32P] orthophosphate were injected intraventricularly into adult female rats. After variable intervals from injection (1–10 min) the animals were sacrificed by means of a microwave apparatus, and phosphorylcholine and choline phosphoglycerides extracted from brain and counted for radioactivity content after separation. The kinetic constants (K) for phosphorylcholine incorporation into lipids were determined both for 32P] and 3H] labeling. From the data obtained by these procedures it is concluded that base-exchange reactions for choline incorporation into lipids are operating in rat brain in vivo and that they represent a rapidly equilibrating system. |
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